TCRab/Vb2 抗体 (SPRD)
Quick Overview for TCRab/Vb2 抗体 (SPRD) (ABIN376730)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- Spectral Red ™
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特异性
- Chicken/Peacock/Guinea Fowl TCRαβ/Vβ2, Mr 48 & 40 kDa
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产品特性
- Mouse Anti-Chicken TCRab/Vb2-SPRD
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纯化方法
- Purified
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免疫原
- CD3+ TCR1- TCR2- Ia- chicken blood mononuclear cells
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亚型
- IgG1
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anti-TCRab/Vb2 antibody (PE)
适用: 小鸡 FACS 宿主: 小鼠 Monoclonal TCR-3 PE
anti-TCRab/Vb2 antibody适用: 小鸡 FACS 宿主: 小鼠 Monoclonal TCR-3 unconjugated
anti-TCRab/Vb2 antibody (Biotin)适用: 小鸡 FACS 宿主: 小鼠 Monoclonal TCR-3 Biotin
anti-TCRab/Vb2 antibody (FITC)适用: 小鸡 FACS 宿主: 小鼠 Monoclonal TCR-3 FITC
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应用备注
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- Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Depletion - Reported in literature
- Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.5 g/106 cells SPRD conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
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说明
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In ovo suppression of the development of TCRab-Vb2 cell
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样本量
- 1 mL
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限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
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Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed. -
储存条件
- 4 °C
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储存方法
- Store at 2-8°C
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- TCRab/Vb2
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背景
- In the chicken, two distinct subpopulations of αβ T cells appear in the thymus subsequent to the appearance of γδ T cells. These subpopulations, originally denoted as TCR2 and TCR3, arise sequentially in the thymus during ontogeny and are now known to represent two distinct V β families, V β 1 and V β 2, respectively. The TCR3 MAb reacts with approximately 9 % of thymocytes, 15-25 % of blood mononuclear cells and 13 % of splenocytes young adult chickens. Two-color immunofluorescence has revealed that the TCR3 + thymocytes include CD4 + CD8 -, CD4 - CD8 +, CD4 + CD8 + and CD4 - CD8 - subpopulations. The TCR3 + thymocytes can be separated into two subsets. One subset is characterized by relatively low levels of expression of the TCR3/CD3 complex and most of the cells in this subset are CD4 + CD8 +. Cells in the other subset express TCR3/CD3 in higher density and are either CD4 + CD8 - or CD4 - CD8 +, corresponding to the more mature medullary subset of thymocytes. The TCR3 + cells in the blood and spleen express relatively high levels of the TCR3/CD3 receptor complex and are “single positive, with CD4 + CD8 - cells being four times more frequent that the CD4 - CD8 + cells (ca. 80 % CD4 + vs ca. 20 % CD8 + ).
抗原
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